# |
PMID |
Sentence |
1 |
9668461
|
The response of PBMC collected from cattle immunized with killed Cowdria to MAP1, MAP1-B, MAP2 and to a lysate of Cowdria was characterized in vitro.
|
2 |
9668461
|
Restimulation of PBMC with MAP1, MAP1-B, and MAP2 proved difficult, and cultures usually stopped proliferating after 2 to 3 weeks.
|
3 |
9668461
|
This study indicates that in animals immunized with killed Cowdria, proteins other than the serologically immunodominant MAP1 and MAP2 are strongly immunogenic for T lymphocytes.
|
4 |
9668461
|
The response of PBMC collected from cattle immunized with killed Cowdria to MAP1, MAP1-B, MAP2 and to a lysate of Cowdria was characterized in vitro.
|
5 |
9668461
|
Restimulation of PBMC with MAP1, MAP1-B, and MAP2 proved difficult, and cultures usually stopped proliferating after 2 to 3 weeks.
|
6 |
9668461
|
This study indicates that in animals immunized with killed Cowdria, proteins other than the serologically immunodominant MAP1 and MAP2 are strongly immunogenic for T lymphocytes.
|
7 |
9668461
|
The response of PBMC collected from cattle immunized with killed Cowdria to MAP1, MAP1-B, MAP2 and to a lysate of Cowdria was characterized in vitro.
|
8 |
9668461
|
Restimulation of PBMC with MAP1, MAP1-B, and MAP2 proved difficult, and cultures usually stopped proliferating after 2 to 3 weeks.
|
9 |
9668461
|
This study indicates that in animals immunized with killed Cowdria, proteins other than the serologically immunodominant MAP1 and MAP2 are strongly immunogenic for T lymphocytes.
|
10 |
11867164
|
We have examined T cell responses against recombinant analogues of the surface-exposed C. ruminantium major antigen 1 (MAP1) a 28.8 kDa protein and MAP2 (21 kDa) antigen in cattle immunised by infection and treatment.
|
11 |
11867164
|
MAP1-specific responses were predominantly restricted to cluster of differentiation four antigen positive T cells (CD4+ T cells).
|
12 |
11867164
|
Reverse transcription polymerase chain reaction (RT-PCR) analysis of cytokine expression by T cell lines derived from this population revealed strong expression of interferon gamma (IFN-gamma), interferon alpha (IFN-alpha), tumour necrosis factor alpha (TNF-alpha), tumour necrosis factor beta (TNF-beta), interleukin-2 receptor alpha (IL-2Ralpha) transcripts, and weak expression of IL-2 and IL-4.
|
13 |
11867164
|
CD4+ T cell clones specific for MAP1 were generated.
|
14 |
11867164
|
RT-PCR analysis of cytokine expression by T cell lines which were dominated by gammadelta T cells revealed expression of IFN-gamma, TNF-alpha, TNF-beta, IL-2Ralpha transcripts.
|
15 |
11867164
|
Our findings indicate that immunisation of cattle by infection with C. ruminantium results in generation of MAP1- and MAP2-specific T cell responses that may play a role in protection against the pathogen.
|
16 |
11867164
|
We have examined T cell responses against recombinant analogues of the surface-exposed C. ruminantium major antigen 1 (MAP1) a 28.8 kDa protein and MAP2 (21 kDa) antigen in cattle immunised by infection and treatment.
|
17 |
11867164
|
MAP1-specific responses were predominantly restricted to cluster of differentiation four antigen positive T cells (CD4+ T cells).
|
18 |
11867164
|
Reverse transcription polymerase chain reaction (RT-PCR) analysis of cytokine expression by T cell lines derived from this population revealed strong expression of interferon gamma (IFN-gamma), interferon alpha (IFN-alpha), tumour necrosis factor alpha (TNF-alpha), tumour necrosis factor beta (TNF-beta), interleukin-2 receptor alpha (IL-2Ralpha) transcripts, and weak expression of IL-2 and IL-4.
|
19 |
11867164
|
CD4+ T cell clones specific for MAP1 were generated.
|
20 |
11867164
|
RT-PCR analysis of cytokine expression by T cell lines which were dominated by gammadelta T cells revealed expression of IFN-gamma, TNF-alpha, TNF-beta, IL-2Ralpha transcripts.
|
21 |
11867164
|
Our findings indicate that immunisation of cattle by infection with C. ruminantium results in generation of MAP1- and MAP2-specific T cell responses that may play a role in protection against the pathogen.
|
22 |
23724014
|
In this study, we investigated a novel neuronal differentiation strategy in vitro with Lmx1α and NTN.
|
23 |
23724014
|
The result showed that cells isolated from the umbilical cord were negative for CD45, CD34 and HLA-DR, but were positive for CD44, CD49d, CD29.
|
24 |
23724014
|
After those cells were infected with recombinant adenovirus, RT-PCR result shows that both Lmx1α and NTN genes were transcribed in hUC-MSCs.
|
25 |
23724014
|
After hUC-MSCs were induced with endogenous and exogenous factors, the mature neurons specific gene TH, Pitx3 was transcripted and the neurons specific protein TH, β-tubulinIII, NSE, Nestin, MAP-2 was expressed in those differentiated cells.
|