# |
PMID |
Sentence |
1 |
2829041
|
Two nonproductive clones, NP-1 and NP-2, were further characterized in comparison with the virus productive cells.
|
2 |
19955324
|
Immunofluorescence assays (IFAs) for detection of human bocavirus (HBoV) proteins (VP1, VP2, NP-1, and NS1) were developed.
|
3 |
19955324
|
Sensitivities of NP-1 and NS1 IFAs were low.
|
4 |
19955324
|
Immunofluorescence assays (IFAs) for detection of human bocavirus (HBoV) proteins (VP1, VP2, NP-1, and NS1) were developed.
|
5 |
19955324
|
Sensitivities of NP-1 and NS1 IFAs were low.
|
6 |
25609691
|
Positive control tracheas were obtained through hyperimmunization of birds with adjuvated NP1 and NP2 peptide conjugated with keyhole limpet hemocyanin administered both orally and parenterally; negative birds received no antigen.
|
7 |
25609691
|
ELISA was performed on NP1- or NP2-positive trachea samples, negative trachea samples, and blank wells with different levels of NP1 and NP2 coating peptides (5 or 10 μg/mL) using two different secondary antibodies (Gene Tex, GT:, or Thermo Scientific, TS:), with or without an acetate wash, and using maximum, medium, or low binding ELISA plates.
|
8 |
25609691
|
These results suggest that the selection of appropriate secondary antibodies, binding plates, and ELISA reagent protocols all play important roles in determining NP1- or NP2-specific IgA levels in trachea samples.
|
9 |
25609691
|
Positive control tracheas were obtained through hyperimmunization of birds with adjuvated NP1 and NP2 peptide conjugated with keyhole limpet hemocyanin administered both orally and parenterally; negative birds received no antigen.
|
10 |
25609691
|
ELISA was performed on NP1- or NP2-positive trachea samples, negative trachea samples, and blank wells with different levels of NP1 and NP2 coating peptides (5 or 10 μg/mL) using two different secondary antibodies (Gene Tex, GT:, or Thermo Scientific, TS:), with or without an acetate wash, and using maximum, medium, or low binding ELISA plates.
|
11 |
25609691
|
These results suggest that the selection of appropriate secondary antibodies, binding plates, and ELISA reagent protocols all play important roles in determining NP1- or NP2-specific IgA levels in trachea samples.
|
12 |
25609691
|
Positive control tracheas were obtained through hyperimmunization of birds with adjuvated NP1 and NP2 peptide conjugated with keyhole limpet hemocyanin administered both orally and parenterally; negative birds received no antigen.
|
13 |
25609691
|
ELISA was performed on NP1- or NP2-positive trachea samples, negative trachea samples, and blank wells with different levels of NP1 and NP2 coating peptides (5 or 10 μg/mL) using two different secondary antibodies (Gene Tex, GT:, or Thermo Scientific, TS:), with or without an acetate wash, and using maximum, medium, or low binding ELISA plates.
|
14 |
25609691
|
These results suggest that the selection of appropriate secondary antibodies, binding plates, and ELISA reagent protocols all play important roles in determining NP1- or NP2-specific IgA levels in trachea samples.
|