Ignet

Gene Information

Gene symbol: PRKDC

Gene name: protein kinase, DNA-activated, catalytic polypeptide

HGNC ID: 9413

Synonyms: DNPK1, p350, DNAPK, XRCC7, DNA-PKcs

Related Genes

#	Gene Symbol	Number of hits
1	CD34	1 hits
2	DCLRE1C	1 hits
3	H2AFX	1 hits
4	HBEGF	1 hits
5	INS	1 hits
6	LIG4	1 hits
7	MRE11A	1 hits
8	NBN	1 hits
9	NCOR2	1 hits
10	PARP1	1 hits
11	PDX1	1 hits
12	RAD50	1 hits
13	TIPARP	1 hits
14	XRCC4	1 hits
15	XRCC5	1 hits
16	XRCC6	1 hits

Related Sentences

#	PMID	Sentence
1	12045092	Recombination is initiated by the lymphoid-specific RAG1 and RAG2 proteins, which cooperate to make double-strand breaks at specific recognition sequences (recombination signal sequences, RSSs).
2	12045092	Broken ends are then processed and joined with the help of several factors also involved in repair of radiation-damaged DNA, including the DNA-dependent protein kinase (DNA-PK) and the Ku, Artemis, DNA ligase IV, and Xrcc4 proteins, and possibly histone H2AX and the Mre11/Rad50/Nbs1 complex.
3	16166097	The transcription factor PDX-1 plays a crucial role during pancreatic development and in the function of insulin-producing beta cells.
4	16166097	These proteins, Ku70 and Ku80, are regulatory subunits of DNA-dependent protein kinase (DNA-PK).
5	16166097	We determined that the interaction between PDX-1 and Ku70 or Ku80 is dependent on the homeodomain of PDX-1.
6	16166097	Most interestingly, we demonstrated in vitro that the DNA-PK phosphorylates PDX-1 on threonine 11.
7	16166097	However, in response to radiation, which activates DNA-PK, a second form of the PDX-1 protein appears rapidly.
8	16166097	In correlation with this degradation, we observed a subsequent reduction in the activation of the insulin promoter and a decrease in PDX-1-mediated gene expression, i.e. glut2 and glucokinase.
9	16166097	Our study demonstrates that radiation, through the activation of DNA-PK, may regulate PDX-1 protein expression.
10	16166097	The transcription factor PDX-1 plays a crucial role during pancreatic development and in the function of insulin-producing beta cells.
11	16166097	These proteins, Ku70 and Ku80, are regulatory subunits of DNA-dependent protein kinase (DNA-PK).
12	16166097	We determined that the interaction between PDX-1 and Ku70 or Ku80 is dependent on the homeodomain of PDX-1.
13	16166097	Most interestingly, we demonstrated in vitro that the DNA-PK phosphorylates PDX-1 on threonine 11.
14	16166097	However, in response to radiation, which activates DNA-PK, a second form of the PDX-1 protein appears rapidly.
15	16166097	In correlation with this degradation, we observed a subsequent reduction in the activation of the insulin promoter and a decrease in PDX-1-mediated gene expression, i.e. glut2 and glucokinase.
16	16166097	Our study demonstrates that radiation, through the activation of DNA-PK, may regulate PDX-1 protein expression.
17	16166097	The transcription factor PDX-1 plays a crucial role during pancreatic development and in the function of insulin-producing beta cells.
18	16166097	These proteins, Ku70 and Ku80, are regulatory subunits of DNA-dependent protein kinase (DNA-PK).
19	16166097	We determined that the interaction between PDX-1 and Ku70 or Ku80 is dependent on the homeodomain of PDX-1.
20	16166097	Most interestingly, we demonstrated in vitro that the DNA-PK phosphorylates PDX-1 on threonine 11.
21	16166097	However, in response to radiation, which activates DNA-PK, a second form of the PDX-1 protein appears rapidly.
22	16166097	In correlation with this degradation, we observed a subsequent reduction in the activation of the insulin promoter and a decrease in PDX-1-mediated gene expression, i.e. glut2 and glucokinase.
23	16166097	Our study demonstrates that radiation, through the activation of DNA-PK, may regulate PDX-1 protein expression.
24	16166097	The transcription factor PDX-1 plays a crucial role during pancreatic development and in the function of insulin-producing beta cells.
25	16166097	These proteins, Ku70 and Ku80, are regulatory subunits of DNA-dependent protein kinase (DNA-PK).
26	16166097	We determined that the interaction between PDX-1 and Ku70 or Ku80 is dependent on the homeodomain of PDX-1.
27	16166097	Most interestingly, we demonstrated in vitro that the DNA-PK phosphorylates PDX-1 on threonine 11.
28	16166097	However, in response to radiation, which activates DNA-PK, a second form of the PDX-1 protein appears rapidly.
29	16166097	In correlation with this degradation, we observed a subsequent reduction in the activation of the insulin promoter and a decrease in PDX-1-mediated gene expression, i.e. glut2 and glucokinase.
30	16166097	Our study demonstrates that radiation, through the activation of DNA-PK, may regulate PDX-1 protein expression.
31	16504547	Down-regulation of PARP-1, but not of Ku80 or DNA-PKcs', results in higher gene targeting efficiency.
32	16504547	To test this hypothesis, we examined gene targeting frequencies (TF) in DNA-PK(cs), Ku80 and poly(ADP-ribose) polymerase (PARP-1) nullizygous cells.
33	16504547	We observed a 3-fold TF increase in PARP-1 knockout embryonic stem (ES) cells, which is consistent with the predicted role of PARP-1 as a switch between HR and NHEJ.
34	16504547	To a lesser extent, such effect could be reproduced upon chemical inhibition of PARP-1.
35	16504547	Down-regulation of PARP-1, but not of Ku80 or DNA-PKcs', results in higher gene targeting efficiency.
36	16504547	To test this hypothesis, we examined gene targeting frequencies (TF) in DNA-PK(cs), Ku80 and poly(ADP-ribose) polymerase (PARP-1) nullizygous cells.
37	16504547	We observed a 3-fold TF increase in PARP-1 knockout embryonic stem (ES) cells, which is consistent with the predicted role of PARP-1 as a switch between HR and NHEJ.
38	16504547	To a lesser extent, such effect could be reproduced upon chemical inhibition of PARP-1.
39	16982777	We unexpectedly found that the corepressor silencing mediator for retinoid and thyroid hormone receptor (SMRT) associates with the DNA-PK repair complex.
40	16982777	The SMRT/histone deacetylase 3 complex is required for the transcriptional repressive property of the Ku70 subunit of the repair complex.
41	23747725	The first strain, C.B-17/Icr-Prkdc(scid)/Prkdc(scid)-INS-TRECK-Tg, carries the diphtheria toxin receptor (hDTR) driven by the human insulin gene promoter, while the other strain, C57BL/6-ins2(BAC)-TRECK-Tg, expresses hDTR cDNA under the control of the mouse insulin II gene promoter.
42	23747725	With regard to the C.B-17/Icr-Prkdc(scid)/Prkdc(scid)-INS-TRECK-Tg strain, only one of three Tg strains exhibited proper expression of hDTR in pancreatic β cells.
43	23747725	Using the C.B-17/Icr-Prkdc(scid)/Prkdc(scid)-INS-TRECK-Tg model, we tried to perform regenerative therapeutic approaches: allogeneic transplantation of pancreatic islet cells from C57BL/6 and xenogeneic transplantation of CD34(+) human umbilical cord blood cells.
44	23747725	Both approaches successfully rescued C.B-17/Icr-Prkdc(scid)/Prkdc(scid)-INS-TRECK-Tg mice from hyperglycemia caused by DT administration.
45	23747725	The first strain, C.B-17/Icr-Prkdc(scid)/Prkdc(scid)-INS-TRECK-Tg, carries the diphtheria toxin receptor (hDTR) driven by the human insulin gene promoter, while the other strain, C57BL/6-ins2(BAC)-TRECK-Tg, expresses hDTR cDNA under the control of the mouse insulin II gene promoter.
46	23747725	With regard to the C.B-17/Icr-Prkdc(scid)/Prkdc(scid)-INS-TRECK-Tg strain, only one of three Tg strains exhibited proper expression of hDTR in pancreatic β cells.
47	23747725	Using the C.B-17/Icr-Prkdc(scid)/Prkdc(scid)-INS-TRECK-Tg model, we tried to perform regenerative therapeutic approaches: allogeneic transplantation of pancreatic islet cells from C57BL/6 and xenogeneic transplantation of CD34(+) human umbilical cord blood cells.
48	23747725	Both approaches successfully rescued C.B-17/Icr-Prkdc(scid)/Prkdc(scid)-INS-TRECK-Tg mice from hyperglycemia caused by DT administration.
49	23747725	The first strain, C.B-17/Icr-Prkdc(scid)/Prkdc(scid)-INS-TRECK-Tg, carries the diphtheria toxin receptor (hDTR) driven by the human insulin gene promoter, while the other strain, C57BL/6-ins2(BAC)-TRECK-Tg, expresses hDTR cDNA under the control of the mouse insulin II gene promoter.
50	23747725	With regard to the C.B-17/Icr-Prkdc(scid)/Prkdc(scid)-INS-TRECK-Tg strain, only one of three Tg strains exhibited proper expression of hDTR in pancreatic β cells.
51	23747725	Using the C.B-17/Icr-Prkdc(scid)/Prkdc(scid)-INS-TRECK-Tg model, we tried to perform regenerative therapeutic approaches: allogeneic transplantation of pancreatic islet cells from C57BL/6 and xenogeneic transplantation of CD34(+) human umbilical cord blood cells.
52	23747725	Both approaches successfully rescued C.B-17/Icr-Prkdc(scid)/Prkdc(scid)-INS-TRECK-Tg mice from hyperglycemia caused by DT administration.
53	23747725	The first strain, C.B-17/Icr-Prkdc(scid)/Prkdc(scid)-INS-TRECK-Tg, carries the diphtheria toxin receptor (hDTR) driven by the human insulin gene promoter, while the other strain, C57BL/6-ins2(BAC)-TRECK-Tg, expresses hDTR cDNA under the control of the mouse insulin II gene promoter.
54	23747725	With regard to the C.B-17/Icr-Prkdc(scid)/Prkdc(scid)-INS-TRECK-Tg strain, only one of three Tg strains exhibited proper expression of hDTR in pancreatic β cells.
55	23747725	Using the C.B-17/Icr-Prkdc(scid)/Prkdc(scid)-INS-TRECK-Tg model, we tried to perform regenerative therapeutic approaches: allogeneic transplantation of pancreatic islet cells from C57BL/6 and xenogeneic transplantation of CD34(+) human umbilical cord blood cells.
56	23747725	Both approaches successfully rescued C.B-17/Icr-Prkdc(scid)/Prkdc(scid)-INS-TRECK-Tg mice from hyperglycemia caused by DT administration.